EVALUATION OF THE EFFECTIVENESS OF DISINFECTANTS FOR DRINKING WATER DECONTAMINATION UNDER EXTREME CONDITIONS

Olena Surmasheva; Olena Polka; Oksana Molchanets; Mykhailo Rosada; Alla Koblianska

doi:10.5281/zenodo.22817171

Authors

Olena Surmasheva State Institution “Marzieiev Institute for Public Health of the National Academy of Medical Sciences of Ukraine”, Kyiv, Ukraine
Olena Polka State Institution “Marzieiev Institute for Public Health of the National Academy of Medical Sciences of Ukraine”, Kyiv, Ukraine
Oksana Molchanets State Institution “Marzieiev Institute for Public Health of the National Academy of Medical Sciences of Ukraine”, Kyiv, Ukraine
Mykhailo Rosada State Institution “Marzieiev Institute for Public Health of the National Academy of Medical Sciences of Ukraine”, Kyiv, Ukraine
Alla Koblianska State Institution “Marzieiev Institute for Public Health of the National Academy of Medical Sciences of Ukraine”, Kyiv, Ukraine

DOI:

https://doi.org/10.5281/zenodo.22817171

Keywords:

disinfectants, microorganisms, specific activity, drinking water

Abstract

Objective. To test methods for determining the specific activity of disinfectants used for drinking water decontamination under extreme conditions and to develop a phased evaluation algorithm aligned with Ukrainian and European requirements.

Methods. Laboratory studies were conducted using quantitative suspension tests and methods simulating practical water-treatment conditions. Six disinfectants with different chemical compositions were evaluated: Eco Chlor, Novochlor-extra, Blanidas-C Hypochlorite, Sanika-Anolyte, Bioluft CL, and SterilOx. Reference strains of Gram-positive and Gram-negative bacteria, bacterial spores, yeasts, and moulds were used to assess bactericidal, sporicidal, yeasticidal, and fungicidal activity. Tests were performed under clean conditions at 20.0 ± 1.0 °C using the concentrations and contact times specified in the study protocols.

Results. Eco Chlor was effective against bacteria and fungi at 0.5% for 15 minutes. Novochlor-extra showed bactericidal and yeasticidal activity at 0.5% for 30 minutes, fungicidal activity at 0.5% for 60 minutes, and sporicidal activity at 2.0% for 60 minutes. Blanidas-C Hypochlorite was effective against bacteria and fungi at active-chlorine concentrations of 0.03% and 0.2% for 15 minutes. Sanika-Anolyte, Bioluft CL, and SterilOx were effective in the tested practical-use regimes; both SterilOx regimes achieved complete decontamination from bacteria and yeast-like fungi. The findings supported a phased algorithm for evaluating disinfectants intended for emergency drinking-water treatment.

Conclusions. The investigated disinfectants demonstrated efficacy in European-standard suspension tests and in conditions simulating practical use. A phased assessment combining basic activity testing, quantitative suspension testing, and practical-use evaluation can support the selection of agents for drinking water decontamination when centralized water supply is unavailable.